Protein Information

Name DAD1
Synonyms DAD 1; DAD1; DAD1 protein; Defender against cell death; Defender against cell death 1; Defender against cell death 1 variant; Dolichyl diphosphooligosaccharide protein glycosyltransferase subunit DAD1; OST 2…

Compound Information

Name jasmonic acid
CAS

Reference List

PubMed Abstract RScore(About this table)
11595796 Ishiguro S, Kawai-Oda A, Ueda J, Nishida I, Okada K: The DEFECTIVE IN ANTHER DEHISCIENCE gene encodes a novel phospholipase A1 catalyzing the initial step of jasmonic acid biosynthesis, which synchronizes pollen maturation, anther dehiscence, and flower opening in Arabidopsis. Plant Cell. 2001 Oct;13(10):2191-209.

We identified the DAD1 gene by T-DNA tagging, which is characteristic to a putative N-terminal transit peptide and a conserved motif found in lipase active sites.
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20348210 Ellinger D, Stingl N, Kubigsteltig II, Bals T, Juenger M, Pollmann S, Berger S, Schuenemann D, Mueller MJ: DGL and DAD1 lipases are not essential for wound- and pathogen-induced jasmonate biosynthesis: redundant lipases contribute to jasmonate formation. Plant Physiol. 2010 Mar 26.

Two sn-1 specific acyl hydrolases, DAD1 and DGL, have been reported to be localized in plastids and to be essential and sufficient for jasmonate biosynthesis in Arabidopsis leaves.
Here we show that levels of 12-oxo-phytodienoic acid (OPDA) and jasmonic acid (JA) in three different DGL-RNAi lines and the dad1 mutant were similar to wild type levels during the early wound response as well as after Pseudomonas infection.
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19704561 Hyun Y, Lee I: Generating and maintaining jasmonic acid in Arabidopsis. Plant Signal Behav. 2008 Oct;3(10):798-800.

Recently we have demonstrated cooperative and differentiated roles of two chloroplast localized galactolipases, DGL (DONGLE) and DAD1 (DEFECTIVE IN ANTHER DEHISCENCE 1), for the regulation of JA content.
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18267087 Hyun Y, Choi S, Hwang HJ, Yu J, Nam SJ, Ko J, Park JY, Seo YS, Kim EY, Ryu SB, Kim WT, Lee YH, Kang H, Lee I: Cooperation and functional diversification of two closely related galactolipase genes for jasmonate biosynthesis. Dev Cell. 2008 Feb;14(2):183-92.

Jasmonic acid (JA) plays pivotal roles in diverse plant biological processes, including wound response.
We report here that DONGLE (DGL), a homolog of DEFECTIVE IN ANTHER DEHISCENCE1 (DAD1), encodes a chloroplast-targeted lipase with strong galactolipase and weak phospholipase A (1) activity.
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20007966 Tabata R, Ikezaki M, Fujibe T, Aida M, Tian CE, Ueno Y, Yamamoto KT, Machida Y, Nakamura K, Ishiguro S: Arabidopsis auxin response factor6 and 8 regulate jasmonic acid biosynthesis and floral organ development via repression of class 1 KNOX genes. Plant Cell Physiol. 2010 Jan;51(1):164-75. Epub 2009 Dec 10.

Among JA biosynthetic genes, the expression level of DAD1 (DEFECTIVE IN ANTHER DEHISCENCE1) was markedly decreased in the double mutant, suggesting that ARF6 and ARF8 are required for activation of DAD1 expression.
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19325888 Cheng H, Song S, Xiao L, Soo HM, Cheng Z, Xie D, Peng J: Gibberellin acts through jasmonate to control the expression of MYB21, MYB24, and MYB57 to promote stamen filament growth in Arabidopsis. PLoS Genet. 2009 Mar;5(3):e1000440. Epub 2009 Mar 27.


Further genetic and molecular studies demonstrate that GA suppresses DELLAs to mobilize the expression of the key JA biosynthesis gene DAD1, and this is consistent with the observation that the JA content in the young flower buds of the GA-deficient quadruple mutant ga1-3 gai-t6 rga-t2 rgl1-1 is much lower than that in the WT.
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