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Valbuena D, Sanchez-Luengo S, Galan A, Sanchez E, Gomez E, Poo ME, Ruiz V, Genbacev O, Krtolica A, Pellicer A, Moreno R, Simon C: Efficient method for slow cryopreservation of human embryonic stem cells in xeno-free conditions. Reprod Biomed Online. 2008 Jul;17(1):127-35.
After thawing, cells retained the inherent undifferentiated characteristics of hESC and growth rate curve, with a stable karyotype, telomerase activity and teratoma formation when injected into severe combined immunodeficient animals, which was comparable with the fresh lines. |
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