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Manning LR, Fantl WJ, Manning JM: Functional measurements on carboxymethylated hemoglobin, a potential blood substitute: effects of sodium chloride on pH values that influence the alkaline Bohr effect. Biomater Artif Cells Artif Organs. 1990;18(2):133-41.
The leakage of chloride from electrodes during measurements of the alkaline Bohr effect of hemoglobin (by the proton release method) amounted to 1-5 mM concentration of the anion depending on the type of electrode employed. This concentration, together with the amount of chloride found to be intrinsically bound to hemoglobin (0.2-0.6 mM), could mask the contribution of chloride to various hemoglobin functions. In addition, the concentration of chloride was found to affect the pH of buffers as measured either with a pH meter or with the dye, cresol red. Thus, for 20 mM phosphate buffer, the pH was lowered almost 0.4 pH units in the presence of 0.30 M chloride. For Tris-acetate buffer, the same concentration of chloride led to an increase in pH of about 0.05 units. |
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