| 19230793 |
Zhao J, Wu D, Zhi J: A novel tyrosinase biosensor based on biofunctional ZnO nanorod microarrays on the nanocrystalline diamond electrode for detection of phenolic compounds. Bioelectrochemistry. 2009 Apr;75(1):44-9. Epub 2009 Feb 3.
A novel tyrosinase biosensor based on biofuncational ZnO nanorod microarrays on the boron-doped nanocrystalline diamond (BDND) substrates was developed. The ZnO nanorod microarrays were firstly deposited on BDND thin film surfaces via a low-temperature solution method, and then ZnO nanorods were functionalized with the mixture of 3-aminopropyltriethoxysilane (APTES) and tetraethoxysilane (TEOS) by a co-condensation approach, then tyrosinase was immobilized to amino-modification ZnO nanorod surfaces by the covalent binding. As-prepared tyrosinase biosensors were used for the detection of phenolic compounds. The tyrosinase-modified BDND electrode gave a linear response range of 1-175, 1-150 and 1-150 microM and sensitivity of 576.2, 339.3 and 287.1 microA mmol (-1) cm (-2) for p-cresol, 4-chlorophenol and phenol, respectively. The low detection limit was estimated to be 0.1, 0.25 and 0.2 microM (s (b)/m=3), respectively. Therefore, the biofunctional ZnO nanorod arrays have potential applications as platforms to immobilize other enzymes and bioactive molecules in biosensors. |
5(0,0,0,5) |