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Kochana J, Gala A, Parczewski A, Adamski J: Titania sol-gel-derived tyrosinase-based amperometric biosensor for determination of phenolic compounds in water samples. Anal Bioanal Chem. 2008 Jun;391(4):1275-81. Epub 2008 Jan 10.
Examination of interference effects.. For detection of phenolic compounds in environmental water samples we propose an amperometric biosensor based on tyrosinase immobilized in titania sol-gel. The analytical characteristics toward catechol, p-cresol, phenol, p-chlorophenol, and p-methylcatechol were determined. The linear range for catechol determination was 2.2 x 10 (-7)-1.3 x 10 (-5) mol L (-1) with a limit of detection of 9 x 10 (-8) mol L (-1) and sensitivity 2.0 x 10 (3) mA mol (-1) L. The influence of sample matrix components on the electrode response was studied according to Plackett-Burman experimental design. The potential interferents Mg (2+), Ca (2+), HCO3 (-), SO4 (2-), and Cl (-), which are usually encountered in waters, were taken into account in the examination. Cu (2+) was also taken into account, because CuSO (4) is sometimes added to a water sample, as a preservative, before determination of phenolic compounds. It was found that among the ions tested only Mg (2+) and Ca (2+) did not directly affect the electrode response. The developed biosensor was used for determination of catechol in spring and surface water samples using the standard addition method. |
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