| 18763820 |
Boyce JP, Brown ME, Chin W, Fitzner JN, Paxton RJ, Shen M, Stevens T, Wolfson MF, Wright CD: Identification of 14-3-3zeta by chemical affinity with salicylanilide inhibitors of interleukin-12p40 production. Bioconjug Chem. 2008 Sep;19(9):1775-84. Epub 2008 Sep 3.
Salicylanilides were found as selective inhibitors of interleukin-12p40 production in stimulated dendritic cells. The conversion of one of these bioactive salicylanilides into a comparably bioactive, chemically labeled derivative was achieved using a facile and systematic functional group derivatization strategy. This resulted in a tool reagent that was then employed in an affinity chromatography approach that resulted in the identification of the protein 14-3-3zeta as having selective affinity for the chromatography matrix that was derivatized with a salicylanilide that inhibited IL-12p40 production. |
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