| 15904965 |
Qu TT, Chen YG, Yu YS, Wei ZQ, Zhou ZH, Li LJ: Genotypic diversity and epidemiology of high-level gentamicin resistant Enterococcus in a Chinese hospital. J Infect. 2006 Feb;52(2):124-30.
OBJECTIVE: To investigate the antibiotics resistance of Enterococcus, the aminoglycoside-modifying enzymes (AME) and homology of high-level gentamicin resistant (HLGR) Enterococcus in clinical specimens for the implementation of effective infection control measures. METHODS: The resistance of 13 antimicrobial agents was determined by Kirby-Bauer (K-B) or agar dilution method. And the HLGR and high-level streptomycin resistant (HLSR) isolates were screened by agar screen. Production of beta-lactamases was tested by the nitrocefin disc method. The aminoglycoside-modifying enzyme genes were detected by polymerase chain reaction (PCR). Pulsed-field gel electrophoresis (PFGE) was used to analyze the homology of HLGR isolates from in-patients. RESULTS: No isolates resistant to linezolid, vancomycin and teicoplanin were found. Ampicillin-resistant isolates did not produce beta-lactamases and 68 HLGR isolates were screened at the rate of 64.2%. The positive rate of aac (6')-Ie-aph (2'')-Ia was 86.8% and 3 isolates had the new AME gene designated aph (2'')-Ie mostly similar to aph (2'')-Id. Among 51 HLGR isolates from in-patients, PFGE grouped 17 Enterococcus faecalis (E. faecalis) isolates into 4 clusters (A-D), and 33 Enterococcus faecium (E. faecium) isolates into 8 clusters (A-H), of which the A cluster is the main. CONCLUSIONS: HLGR has become the important antibiotic resistance pathogen causing nocosomial infection. And the aac (6')-Ie-aph (2'')-Ia gene was the main aminoglycoside-modifying enzyme gene leading to HLGR. |
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