| 18201202 |
Aoki H, Xu J, Emili A, Chosay JG, Golshani A, Ganoza MC: Interactions of elongation factor EF-P with the Escherichia coli ribosome. FEBS J. 2008 Feb;275(4):671-81. Epub 2008 Jan 12.
EF-P (eubacterial elongation factor P) is a highly conserved protein essential for protein synthesis. We report that EF-P protects 16S rRNA near the G526 streptomycin and the S12 and mRNA binding sites (30S T-site). EF-P also protects domain V of the 23S rRNA proximal to the A-site (50S T-site) and more strongly the A-site of 70S ribosomes. We suggest that EF-P: (a) may play a role in translational fidelity and (b) prevents entry of fMet-tRNA into the A-site enabling it to bind to the 50S P-site. We also report that EF-P promotes a ribosome-dependent accommodation of fMet-tRNA into the 70S P-site. |
31(0,1,1,1) |