| 11583853 |
Gehin G, Bonaly R, Coulon J: The role of glucose in the Kluyveromyces bulgaricus flocculation phenomenon: transduction by cAMP-dependent protein kinase pathway?. FEMS Microbiol Lett. 2001 Sep 25;203(2):229-33.
Yeast flocculation appears to be dependent on several culture conditions such as nitrogen or carbon sources. In 0.2% glucose medium Kluyveromyces bulgaricus flocculation intensity is weak (10% at maximum) by comparison with flocculation in 2% glucose medium (85% maximum). Addition of glucose to K. bulgaricus in exponential growth phase in 0.2% glucose medium produced a rapid increase of the flocculation percentage during the 30 min following the addition of glucose. cAMP and 2,4-dinitrophenol showed similar effects while cAMP-dependent protein kinase (PKA) inhibitors exhibited an antagonist effect. Moreover, the induction of flocculation did not seem to imply translation of new proteins: cycloheximide had no effect, although growth was inhibited. The induction of flocculation mainly implies ATP hydrolysis for activation or secretion of galactose-specific receptors as demonstrated by treatment with NaN (3). We propose a hypothesis that involves a PKA transduction signal leading to flocculation. |
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