| 12903423 |
Zhang L, Li L, Ban L, An W, Liu S, Li X, Xue B, Xu Y: [Effect of sodium azide on mitochondrial membrane potential in SH-SY5Y human neuroblastoma cells]. Zhongguo Yi Xue Ke Xue Yuan Xue Bao. 2000 Oct;22(5):436-9.
OBJECTIVE: To study the role of mitochondrial deficiency in the pathogenesis of neurodegenerative disease by investigating the energy metabolism in a sodium azide inhibited cytochrome-c oxidase SH-SY5Y Cell model. METHODS: Human neuroblastoma SH-SY5Y Cells were exposed to sodium azide, then mitochondrial complex IV activity was assayed by microassay method; cell viability was measured by Thiazolyl blue (MTT) method; mitochondrial membrane potential (MMP) was detected by confocal microscopy and flow cytometry. RESULTS: Cultured SH-SY5Y cells were exposed to 16-64 mmol/L sodium azide for 1 hour, the mitochondrial complex IV activity decreased dose-dependently. MTT absorbance decreased does- and time-dependently in cultured nerve cells treated by 16-128 mmol/L sodium azide for 1-8 hours. After the treatment of 16 mmol/L sodium azide for 1 hour, both the fluorescence intensity of MMP and normal cell events reduced. Decrease of MMP was significant especially in cell processes. CONCLUSION: Sodium azide induced the impairment of mitochondrial energy synthesis in the cultured nerve cells which is an important cause in cell death. |
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