| 2739687 |
Lodovici M, Grassi M, Dolara P: In vitro DNA binding of 2-amino-3-methylimidazo (4,5-f)[5-3H] quinoline (IQ) is modulated by sulfotransferase and sulfate levels. Mutat Res. 1989 Jul;223(3):321-4.
DNA binding in vitro was measured with 2-amino-3- methylimidazo (4,5-f)[5-3H] quinoline (3H-IQ) in the presence of S9 and microsomes from Wistar rat liver. DNA binding of 3H-IQ was catalyzed by both microsomes and S9 and was increased 5-fold in Aroclor (PCB)-pretreated animals. DNA binding was reduced by the specific inhibitor of sulfotransferase 2,6-dichloro-4-nitrophenol and slightly increased by 3'-phosphoadenosine 5'-phosphosulfate. The incubation of IQ in media with increasing concentrations of sulfate produced a modest dose-dependent increase in DNA binding. Liver S9 obtained from rats which had been administered 1% sulfite in drinking water catalyzed a higher binding of IQ to DNA, and this effect was inhibited by pentachlorophenol. The data demonstrate that sulfotransferase has a role in the activation of IQ and that variations in the activity of this enzyme induced by dietary changes may vary the genotoxic activity of IQ. |
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