| 19060947 |
Parfinovich EV, Mochalova LV, Molotkovskii IuG, Bovin NV, Vodovozova EL: [Identification of a new carbohydrate-binding site of influenza virus] . Bioorg Khim. 2008 Sep-Oct;34(5):716-20.
It has recently been shown that the influenza virus can specifically bind the residue of a nonsialylated sulfated oligosaccharide Gal (6SO (3) H) beta1-4GlcNAcbeta (6'SLacNAc). To identify by photoaffinity labeling the virion component that binds 6'SLacNAc, we synthesized a carbohydrate probe containing a (125) I labeled diazocyclopentadien-2-yl carbonyl group as an aglycone. According to the electrophoretic data, the labeled areas corresponded to a large hemagglutinin subunit, a nucleocapsid protein, and neuraminidase (NA). Probing in the presence of an excess of 6'SLacNAcbeta-OCH (2) CH (2) NHAc glycoside resulted in redistribution of the labeling intensity, with the maximum inhibition being observed for NA. The data obtained indicate that NA is a viral 6'SLacNAc-binding protein. |
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