| 15387458 |
Zamora T, Hidalgo C, Lopez FJ, Hernandez F: Determination of fungicide residues in fruits by coupled-column liquid chromatography. J Sep Sci. 2004 Jun;27(9):645-52.
Coupled-column liquid chromatography with fluorescence detection was applied to the determination of o-phenylphenol and bitertanol residues in orange and banana fruits. After extraction with a mixture of acetone, dichloromethane-petroleum ether, and ethyl acetate, an extract aliquot of 100 microL was injected directly without any additional clean-up into the chromatographic system using two reversed phase C18 coupled columns. The LC-LC approach allowed automated sample clean up of the vegetal extracts, leading to a simple and rapid analytical procedure, with limits of quantification between 0.01 and 0.05 mg kg (-1). Recovery experiments performed on orange and banana samples fortified at different concentrations (0.01 - 4 mg kg (-1)) gave average recoveries between 70 and 113% with relative standard deviations lower than 15%. The procedure developed was finally applied to orange and banana samples from different geographical locations and the results were confirmed by GC-MS. |
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