| 9020064 |
Xi L, Squires CH, Monticello DJ, Childs JD: A flavin reductase stimulates DszA and DszC proteins of Rhodococcus erythropolis IGTS8 in vitro. Biochem Biophys Res Commun. 1997 Jan 3;230(1):73-5.
Rhodococcus erythropolis IGTS8 is a gram positive bacterium, which can catabolize dibenzothiophene to 2-hydroxybiphenyl and inorganic sulfur without the cleavage of carbon-carbon bonds. Three structural genes, dszA, dszB, and dszC, have been cloned and shown to be necessary for this phenotype. Here, we demonstrate that a FMN:NADPH oxidoreductase from Vibrio harveyi complements activities of purified DszA and DszC proteins. Furthermore, we propose that DszA and DszC are oxygenase units that do not use NAD (P) H directly, but instead use FMNH2 from a FMN:NADPH oxidoreductase for oxygenation. |
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