| 16054682 |
Lord MS, Stenzel MH, Simmons A, Milthorpe BK: The effect of charged groups on protein interactions with poly (HEMA) hydrogels. Biomaterials. 2006 Feb;27(4):567-75. Epub 2005 Jul 28.
Proteins, lipids and other biomolecules interact strongly with the acrylic-based biomaterials used for contact lenses. Although hydrogels are nominally resistant to protein fouling, many studies have reported considerable amounts of protein bound to poly (2-hydroxyethylmethacrylate) (PHEMA) lenses. This study examined the binding of a series of biomolecules (tear protein analogues, mucin and cholesterol) to poly (methylmethacrylate) (PMMA) and three HEMA-based hydrogels (PHEMA, HEMA plus methacrylic acid (P (HEMA-MAA)), HEMA plus methacrylic acid plus N-vinylpyrrolidone (P (HEMA-MAA-NVP))) by use of a quartz crystal microbalance with dissipation (QCM-D) monitoring. The QCM-D estimates changes in the mass and viscous constant for the adsorbed layer through measurements of frequency and dissipation. Protein interaction with each of the test materials caused a net increase in mass of the material indicating protein binding except for lysozyme interacting with P (HEMA-MAA). A net decrease in mass was observed for lysozyme interacting with P (HEMA-MAA) which may be ascribed to lysozyme collapsing the hydrogel by expelling water. A net mass decrease was observed for cholesterol interacting with each of the hydrogel materials, while a mass increase was observed on PMMA. |
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