| 16534746 |
Requejo R, Tena M: Maize response to acute arsenic toxicity as revealed by proteome analysis of plant shoots. Proteomics. 2006 Apr;6 Suppl 1:S156-62.
Aerial parts (shoots) of maize seedlings fed hydroponically with 300 muM sodium arsenate [As (V)] or 250 muM sodium arsenite [As (III)] for 24 h were analyzed for differentially expressed proteins by 2-DE and digital image analysis. About 15% of total detected proteins (74 out of 500) were up- or, mainly, down-regulated by arsenic, among which 14 were selected as being those most affected by the metalloid. These proteins were analyzed by MALDI-TOF MS and 7 of them were identified: translation initiation factor eIF-5A, ATP synthase, cysteine synthase, malate dehydrogenase, protein kinase C inhibitor, Tn10 transposase-like protein, and guanine nucleotide binding protein. Each of these proteins was completely repressed by As (V) and/or As (III), except protein kinase C inhibitor, which was newly detected after exposure to As (V). |
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