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Yang P, Liu QZ, Li AP, Li T, Hong X, Zhou JW: [The relationship between hormesis of proliferation and oxidative stress induced by sodium arsenite in human embryo lung fibroblasts]. Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi. 2006 Feb;24(2):103-5.
OBJECTIVE: To investigate the relationship between the hormesis of proliferation and oxidative stress induced by sodium arsenite (Na (2) AsO (2)) in human embryo lung fibroblasts (HELF). METHODS: HELF were treated with Na (2) AsO (2) of 0.0, 0.1, 0.5, 1.0, 5.0 and 10.0 micromol/L for 4 hours or 24 hours, respectively. The cell proliferation, the reactive oxygen species (ROS) level, the malondialdehyde (MDA) content and the activity of glutathione peroxide (GSH-Px) and the superoxide dismutase (SOD) in HELF were detected respectively. RESULTS: The HELF proliferation induced by 0.1 and 0.5 micromol/L Na (2) AsO (2) was significantly higher than that in the control group (P < 0.01). The HELF proliferation induced by 5.0 and 10.0 micromol/L Na (2) AsO (2) was significantly lower than that in the control group (P < 0.01) with the dose-effect relation of an inverted U curve. The ROS level induced by Na (2) AsO (2) of between 0.5 and 10.0 micromol/L was significantly increased (P < 0.05, P < 0.01). The positive correlation was found between the ROS level and the exposure dose of Na (2) AsO (2) (r = 0.934, P < 0.01). The 5.0 and 10.0 micromol/L Na (2) AsO (2) induced the significant increase of the MDA contents (P < 0.01) and the significant decrease of the GSH-Px activity compared to those in the control group (P < 0.01). The SOD activity in 0.5 micromol/L Na (2) AsO (2) group was significantly higher than that in the control group (P < 0.01) while the SOD activity induced by 5.0 and 10.0 micromol/L Na (2) AsO (2) was significantly decreased (P < 0.01) if compared with the control group with the dose-effect relation of an inverted U curve. CONCLUSION: The sodium arsenite can induce the hormesis of proliferation in HELF with the dose-effect relation of an inverted U curve. The mechanisms probably relates to different levels of oxidative stress induced by sodium arsenite of different concentrations. |
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