| 17096945 |
Sun XC, Piao FY, Wang Y, Xu YY, Li X, Li B, Jin YP, Sun GF: [Effects of sodium arsenite on catalase in human keratinocytes] . Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi. 2006 Oct;24(10):605-7.
OBJECTIVE: To evaluate the effects of sodium arsenite on the activity, the mRNA and the protein expression of CAT in human keratinocyte cell line (HaCaT). METHODS: The activity of catalase (CAT) was detected by ultraviolet direct velocity assay. RT-PCR was used to detect the mRNA expression of CAT and Western blotting was conducted to detect the protein expression of CAT. RESULTS: If the cells were treated with higher than 5.0 micromol/L sodium arsenite, the activity, mRNA and protein expression of CAT were decreased significantly and in a dosage dependent fashion (P < 0.05). CONCLUSION: CAT is inhibited by sodium arsenite in the transcription, translation and activity levels. |
7(0,0,1,2) |