| 19403408 |
Ying SS, Li X, Huang ML, Meng Y, Zhang ZS: [Effect of angiotensin1-7 on alpha-smooth muscle actin protein expression in rat hepatic stellate cells]. Nan Fang Yi Ke Da Xue Xue Bao. 2009 Apr;29(4):732-3.
OBJECTIVE: To investigate the effect of angiotensin II and angiotensin1-7 on alpha-smooth muscle actin (alpha-SMA)-induced Ca (2+)-independent pathways mediated by Rho kinase2 in hepatic stellate cells (HSCs). METHODS: HSC-T6 cells were treated with 10 micromol/L of AngII, Ang1-7, AngII +Ang1-7, and Ang1-7+A779. RT-PCR was used to detect the expression of Rho kinase2 (Rock2) in Ca (2+)-independent pathways, and alpha-SMA protein expression was detected by Western blotting. RESULTS: The mRNA expression of Rock2 increased significantly in the cells after AngII treatment (P <0.01), but decreased following Ang1-7 treatment. Ang1-7 treatment significantly reduced alpha-SMA level in AngII-induced cells (P <0.01). CONCLUSION: Ang1-7 can inhibit AngII-induced activation of Rock2 and reduce alpha-SMA expression in HSCs. |
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