| 18942088 |
Wendling O, Bornert JM, Chambon P, Metzger D: Efficient temporally-controlled targeted mutagenesis in smooth muscle cells of the adult mouse. Genesis. 2009 Jan;47(1):14-8.
To generate temporally-controlled targeted somatic mutations selectively and efficiently in smooth muscles, we have established a transgenic SMA-Cre-ER (T2) mouse line in which the expression of the Tamoxifen-dependent Cre-ER (T2) recombinase is under the control of a large genomic DNA segment of the mouse smooth muscle alpha actin (SMA) gene, contained in a Bacterial artificial chromosome (Bac). In this transgenic mouse line, Cre-ER (T2)-mediated recombination of LoxP-flanked target DNA is strictly Tamoxifen-dependent, and efficient in both vascular and visceral smooth muscle cells. Moreover, with the exception of few cardiomyocytes, LoxP-flanked DNA excision is restricted to smooth muscle cells. Thus, SMA-Cre-ER (T2) mice should be of great value to analyze gene function in smooth muscles, and to establish new animal models of human smooth muscle disorders. |
112(1,2,2,2) |