| 20001597 |
Goussetis E, Spiropoulos A, Theodosaki M, Stefanaki K, Petrakou E, Graphakos S: Myofibroblasts generated in culture from sclerotic skin lesions of a patient with extensive chronic graft-versus-host disease after allogeneic hematopoietic stem cell transplantation are of recipient origin. Stem Cells Dev. 2009 Dec 11.
The origin (recipient/donor) of the myofibroblasts mediating fibrosis in sclerodermatous cGvHD was investigated. Sclerodermatous specimens obtained from a patient with extensive cGvHD after an HLA-identical sibling bone marrow transplantation were cultured in order to derive tissue myofibroblasts. All proliferating a-SMA+ fibroblastoid cells revealed recipient origin as examined by variable number tandem repeat (VNTR)-PCR. This case report shows that fibrosis in sclerodermatous lesions results from the activation and proliferation of locally-derived recipient fibroblasts rather than from donor-derived fibroblasts or circulating fibrocytes. |
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