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Wang N, Yang J, Yu X, Hu J, Xing C, Ju X, Shen X, Qian J, Zhao X, Wang X: Radial artery calcification in end-stage renal disease patients is associated with deposition of osteopontin and diminished expression of alpha-smooth muscle actin. Auris Nasus Larynx. 2009 Dec;36(6):725-8. Epub 2009 May 23.
AIM: Vascular access is the lifeline of haemodialysis patients and radial-cephalic fistula is the preferred type of access. We investigated vascular calcification in uraemia radial arteries and compared it with clinical parameters. METHODS: Artery specimens from 30 end-stage renal disease patients were collected, examined calcification by von Kossa and Alizarin red staining. Expression of alpha-smooth muscle actin (alpha-SMA) and the main component of bone matrix, osteopontin (OP) were detected by immunohistochemistry. RESULTS: In uraemia vessels, calcification was mainly located in the medial layer. Nineteen (63.33%) patients had no evidence of calcification, six (20%) had mild/moderate calcification and five (16.66%) had severe calcification. Upregulation of OP and diminished expression of alpha-SMA occurred in the medial layer, especially in the area of severe calcification. The calcification score, decreased expression of alpha-SMA and upregulation of OP were positively correlated with older age, serum calcium, serum phosphorus and calcium x phosphorus product (P < 0.01). CONCLUSION: Vascular calcification in uraemia radial arteries is mainly located in the media layer. The risk factors appear to be older age, an elevated serum level of phosphorus, calcium and calcium x phosphorus product. |
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