| 10751587 |
Tsakiris S, Angelogianni P, Schulpis KH, Stavridis JC: Protective effect of L-phenylalanine on rat brain acetylcholinesterase inhibition induced by free radicals. Clin Biochem. 2000 Mar;33(2):103-6.
OBJECTIVE: To investigate whether the preincubation of brain homogenates with L-phenylalanine (Phe) could reverse the free radical effects on brain acetylcholinesterase (AChE) activity, since it has been reported that Phe binds hydroxyl radicals ((*) OH). DESIGN AND METHODS: Two well established systems were used for production of free radicals: (a) FeSO (4) (84 microM) plus ascorbic acid (400 microM), and (b) FeSO (4), ascorbic acid and H (2) O (2) (1 mM) at 37 degrees C in homogenates of adult rat whole brain. Changes in brain AChE activity were studied in the presence of each system separately. RESULTS: AChE was inhibited (18-28%) by both systems of free radicals. This inhibition was reversed when the brain homogenate was preincubated with Phe 1.8 mM. CONCLUSIONS: In accordance with our previous reports, Phe could protect against the direct action of (*) OH radicals on brain AChE and in this way it might be useful in the prevention of certain cholinergic neural dysfunctions. |
5(0,0,0,5) |