| 20153310 |
Gao W, Xie Q, Jin J, Qiao T, Wang H, Chen L, Deng H, Lu Z: HPLC-FLU detection of cortisol distribution in human hair. . Clin Biochem. 2010 Feb 10.
OBJECTIVE: Recently cortisol level in hair is supposed to be a good biomarker of chronic stress. We had developed a new method based on HPLC-FLU for identification of cortisol in human hair and measured cortisol distribution of each 1-cm segment along hair shaft. DESIGN AND METHODS: Hair sample was pulverized and incubated in 0.1 M HCl and extracted with ethyl acetate. The extraction was derived with sulfuric acid. The derivative was extracted with SPE C18 column. RESULTS: The detection limit was 1 pg/mg, the intraday and interday precisions were 7.3% and 8.5%, and average recovery was 102.5+/-13.0%. Cortisol concentrations of 32 humans' hair ranged from 3.28 to 24.83 pg/mg. There was a distinct decline in the cortisol of 1-cm segment along hair shaft. CONCLUSIONS: This method showed that the precision was matched with mass spectroscopy. The cortisol in 1-cm hair segment sensitively reflects a retrospective calendar over 1 month. |
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