| 18968938 |
Vedrine C, Fabiano S, Tran-Minh C: Amperometric tyrosinase based biosensor using an electrogenerated polythiophene film as an entrapment support. Talanta. 2003 Mar 1;59(3):535-44.
An amperometric enzyme sensor using tyrosinase, also called polyphenol oxidase (PPO), was constructed for determination of phenolic compounds and herbicides. The enzyme was entrapped in a conducting polymer, poly 3,4-ethylenedioxythiophene (PEDT), electrochemically generated on a glassy carbon electrode. Several experimental parameters in the electropolymerisation process and working conditions were determined to optimise biosensor performances. Mono-phenol and di-phenol were tested in oxygenated solutions, by amperometric measurements at -200 mV (vs. SCE) in a batch system. The limit of detection of these molecules ranges from 5 to 500 nM. Detection of herbicides was obtained from the inhibition of tyrosinase electrode responses. The limit of detection for atrazine and diuron was 1 and 0.5 mgl (-1) respectively. These data suggest that PEDT film is a promising PPO immobilisation method. |
3(0,0,0,3) |