| 10755368 |
Paci A, Rieutord A, Guillaume D, Traore F, Ropenga J, Husson HP, Brion F: Quantitative high-performance liquid chromatographic determination of acrolein in plasma after derivatization with Luminarin 3. J Chromatogr B Biomed Sci Appl. 2000 Mar 10;739(2):239-46.
A rapid, sensitive and specific high-performance liquid chromatographic method for the quantification of acrolein (1), one of the toxic metabolites of oxazaphosphorine alkylating agents (cyclophosphamide and ifosfamide) was developed. Condensation of acrolein with Luminarin 3 afforded a fluorescent derivative that could be specifically detected and quantified. Chromatographic conditions involved a C18 RP column Uptisphere and a gradient elution system to optimize resolution and time analysis. The method showed high sensitivity with a limit of detection of 100 pmol/ml and a limit of quantification of 300 pmol/ml. This technique is particularly suitable for pharmacokinetic studies on plasma of oxazaphosphorine-receiving patients. |
1(0,0,0,1) |