| 19032949 |
Yoshida M, Tomitori H, Machi Y, Hagihara M, Higashi K, Goda H, Ohya T, Niitsu M, Kashiwagi K, Igarashi K: Acrolein toxicity: Comparison with reactive oxygen species. Biochem Biophys Res Commun. 2009 Jan 9;378(2):313-8. Epub 2008 Nov 24.
The toxicity of acrolein was compared with that of reactive oxygen species using a mouse mammary carcinoma FM3A cell culture system. Complete inhibition of cell growth was accomplished with 10 microM acrolein, 100 microM H (2) O (2), and 20 microM H (2) O (2) plus 1mM vitamin C, which produce () OH, suggesting that toxicity of acrolein is more severe than H (2) O (2) and nearly equal to that of () OH, when these compounds were added extracellularly. Acrolein toxicity was prevented by N-acetyl-l-cysteine and N-benzylhydroxylamine, and attenuated by putrescine and spermidine. Toxicity of H (2) O (2) was prevented by glutathione peroxidase plus N-acetyl-l-cysteine, pyruvate, catalase, and reduced by polyphenol, and toxicity of () OH was prevented by glutathione peroxidase plus N-acetyl-l-cysteine, pyruvate, catalase and reduced by N-acetyl-l-cysteine. The results indicate that prevention of cell toxicity by N-acetyl-l-cysteine was more effective with acrolein than with () OH. Protein and DNA synthesis was damaged primarily by acrolein and reactive oxygen species, respectively. |
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