| 16996028 |
Isoda K, Arita E, Kojima M, Ikkaku M, Tashiro F, Yamato E, Miyazaki J, Kawase M, Kondoh M, Yagi K: Protection against CCl4-induced injury in liver by adenovirally introduced thioredoxin gene. Biochem Biophys Res Commun. 2006 Nov 10;350(1):157-61. Epub 2006 Sep 15.
Antioxidation therapy is a promising strategy for treating or preventing oxidative stress-related liver diseases. The human thioredoxin (TRX) gene was inserted into an adenovirus vector (Adv-TRX), which was administered to mice. The mice were treated with 1 ml/kg CCl4 48 h after the infection. Blood samples were taken and the liver was excised 24 h after the CCl4 treatment. Serum ammonia, aspartate aminotransferase (AST), and alanine aminotransferase (ALT) levels were determined, and liver sections were stained with hematoxylin and eosin. RT-PCR analysis showed that the introduced TRX gene was expressed only in the liver. Adv-TRX decreased the serum ammonia, AST, and ALT levels. Hematoxylin-eosin staining indicated that the CCl4-induced injury was significantly prevented by the Adv-TRX infection. The gene delivery of TRX, which plays a central role in intracellular redox control, was shown to be effective in protecting the liver against oxidative stress-induced injury. |
1(0,0,0,1) |