| 18361832 |
Li J, Cheng LF, Li Z, Cong YW: [Intestinal intraepithelial lymphocytes in cirrhosis: experiment with rats]. Zhonghua Yi Xue Za Zhi. 2008 Jan 22;88(4):233-5.
OBJECTIVE: To investigate the phenotypes of T lymphocytes in the intestinal epithelium in cirrhosis. METHODS: Forty Wistar rats were randomly divided into 2 groups: cirrhosis group (n = 25) undergoing subcutaneous injection of 40% carbon tetrachloride twice a week for 10-12 weeks to establish cirrhosis models, and control group (n = 15). Twelve weeks later the rats were killed with their levers taken out. The T lymphocytes in the intestinal epithelium were isolated and labeled with mouse anti-rat CD3, CD4 and CD8 monoclonal antibody, Flow cytometry was performed. RESULTS: The number of intestinal intraepithelial lymphocytes of the cirrhosis group was (3.4 +/- 1.1) x 10 (5)/cm, significantly higher than that of the control group [(2.3 +/- 0.5) x 10 (5)/cm, P < 0.05]. The proportion of CD3 (+) cells of the cirrhosis group were (76 +/- 8)%, not significantly different from that of the control group [(80 +/- 6)%, P > 0.05]. There were no significant different between two group (P > 0.05). The proportion of CD4 (+) CD8 (+) subpopulation of the cirrhosis group was (6.9 +/- 3.3)%, significantly higher than that of the control group [(3.7 +/- 1.8)%, P < 0.05]. The proportion of CD4 (+) CD8 (-) subpopulation of the cirrhosis group was (6.9 +/- 3.0)%, significantly higher than that of the control group [(4.4 +/- 1.4)%, P < 0.05]. CONCLUSION: There were alterations of the immune barrier function in cirrhosis. The increase of the proportion of CD4 (+) CD8 (+) subpopulation may be associated with the increases of the number of intestinal bacteria. |
4(0,0,0,4) |