| 20175234 |
Zhao SH, Chu YL, Liu EQ: [Expression of keratin 8 in carbon tetrachloride-induced liver injury of mice.]. Zhejiang Da Xue Xue Bao Yi Xue Ban. 2010 Jan;39(1):37-42.
OBJECTIVE: To investigate the expression of keratin 8 (K8) in carbon tetrachloride (CCl (4))-induced liver injury of mice. METHODS: Forty ICR mice were divided into four groups. CCl (4) 300 mu l/kg body weight in olive oil was injected intraperitoneally for 0, 2, 4, 6 weeks in group A, B, C and D, respectively. Mice were sacrificed 3 d after the last injection and then the vital organs were collected and weighed. RT-PCR and immunohistochemistry methods were used to analyze the expression of keratin 8 in the liver. RESULTS: The ratios of liver and body weight were increased significantly after administration of CCl (4), which were 5.60 %, 6.87%, 7.83 % and 7.76% at 0, 2, 4 and 6 weeks after injection, respectively. The expression of K8 was increased at the 2 w, 4 w and 6 w after CL (4) administration. Conclusion: The expression of K8 is positively correlated with the liver injury induced by CCl (4). The accumulation of K8 may be involved in the mechanism of liver injury. |
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