15465306 |
Almeida NL, Oliveira CL, Torriani IL, Loh W: Calorimetric and structural investigation of the interaction of lysozyme and bovine serum albumin with poly (ethylene oxide) and its copolymers. Colloids Surf B Biointerfaces. 2004 Oct 10;38(1-2):67-76. This work reports investigations aiming at verifying the occurrence of specific interactions between lysozyme or bovine serum albumin (BSA) and poly (ethylene oxide) and its copolymers with poly (propylene oxide). Thermal stability of these proteins, followed by means of high sensitivity DSC, was found to be mostly unaffected by the presence of these polymers. Chromatographic experiments (reverse-phase HPLC and size exclusion chromatrography) did not reveal any sign of specific interaction for these mixtures, either. Isothermal titration calorimetry revealed an increase in enthalpy for the mixtures, represented by a positive enthalpy of transfer for these proteins from buffer to polymer solutions. Moreover, SAXS analyses confirmed that at ambient temperatures these polymers do not affect lysozyme structure. In summary, no evidence is found to support earlier suggestions that some kind of complex could be formed between these proteins and poly (ethylene oxide) or its copolymers, but the present results suggest the occurrence of entropically driven hydrophobic effects. |
112(1,2,2,2) |