| 2753027 |
Manabe S, Kanai Y, Wada O: Exposure level monitor of 3-amino-1,4-dimethyl-5H-pyrido [4,3-b] indole, a dietary carcinogen, in rabbits. Environ Mol Mutagen. 1989;14(1):34-41.
The present investigation describes a method for the detection of minute amounts of 3-amino-1,4-dimethyl-5H-pyrido [4,3-b] indole (Trp-P-1), a carcinogenic tryptophan pyrolysate, bound to the hemoglobin of erythrocytes and plasma from rabbits dosed orally with the dietary carcinogen. The method consists of the acid-induced release of the dietary carcinogen adducts as the free carcinogen and their extraction with methylene chloride and subsequent quantitation by high-performance liquid chromatography (HPLC). With this method, Trp-P-1 levels in plasma, platelets, and red blood cells were monitored for 12 weeks to determine a suitable indicator for estimating the exposure levels of the dietary carcinogen. The results suggest that Trp-P-1 in red blood cells that bound covalently to the hemoglobin is the most suitable for monitoring the long-term exposure levels. |
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