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Lou K, Bowers J: Electroimmunoquantitation of serum proteins cross-linked by glutaraldehyde. J Immunol Methods. 1975 Nov;9(1):69-79.
Electroimmunoassay is a simple, rapid and accurate method for quantitating the serum proteins. By use of glutaraldehyde, intermolecular cross-linkage of immunoglobulins to albumin was effected. The conjugation resulted in increased electrophoretic mobility of the immunoglobulins without perceptible change in antigenic determinants. With a Tris-EDTA-boric acid buffer system, the cross-linked immunoglobulins migrated anodically in an electrical field. Six serum proteins from each of 20 samples were quantitated by electroimmunoassay, and the results correlated with values obtained by radial immunodiffusion. |
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