| 10793709 |
Kato AC, Borstad L, Fraser MJ, Denhardt DT: Isolation of repeated and self-complementary sequences from E. coli DNA. Nucleic Acids Res. 1974 Nov;1(11):1539-48.
We have used the single-strand specific nuclease from Neurospora crassa and chromatography on methylated albumin-kieselguhr to purify and characterize repeated and self-complementary sequences from Escherichia coli DNA. Approximately 0.5% of the genome renatures spontaneously at zero time and another 2% renatures somewhat more rapidly than the total DNA. The early renaturing DNA has a base composition and a Tm similar to the total DNA and contains on the average 100 base pairs; the self-complementary DNA also has a base composition like E. coli but contains a mean of 170 base pairs. No evidence was obtained for the presence of a highly redundant sequence. |
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