| 15807124 |
Yan X, Sin Y, Yu X, Zhang W, Jin M: [Analysis of low-polarity constituents of Reniochalina sp. by gas chromatography-mass spectrometry]. Se Pu. 2004 Nov;22(6):652-4.
The sponge of Reniochalina sp. was collected from China Yellow Sea. The specimen (dry weight 100 g) was repeatedly extracted with cyclohexane. The combined crude extract was then evaporated in vacuo, and the dark red residue (5 g) was subjected to a silica gel column for chromatographic separation using gradient mixtures of ethyl acetate and cyclohexane as eluents. The fraction eluted with ethyl acetate-cyclohexane (1:9, v/v) was repeatedly separated by preparative liquid chromatography (HiQ sil C18-10 column, 250 mm x 21.2 mm i.d. ) to afford sample B and sample C as dark red oil. These two low-polarity samples were analyzed by gas chromatography-mass spectrometry (GC-MS) equipped with an HP-5 column (30 m x 0.25 mm i.d.). The oven temperature was raised from 40 degrees C to 250 degrees C at 10 degrees C /min and kept at 250 degrees C for 70 min. Using GC-MS coupled with 1H-nuclear magnetic resonance (1H-NMR), 18 compounds including alkanes, alkenes, fatty acids, fatty acid esters, phthalic acid and phthalic esters, were identified. The results provide to be helpful for in taxonomy of Reniochalina. |
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