| 19344927 |
Luo J, Ma M, Zha J, Wang Z: Characterization of aryl hydrocarbon receptor agonists in sediments of Wenyu River, Beijing, China. Water Res. 2009 May;43(9):2441-8. Epub 2009 Mar 20.
Aryl hydrocarbon receptor agonistic (Ah-agonistic) effects of 23 sediments from Wenyu River in Beijing, China were evaluated using the H4IIE cell bioassay. Five samples were selected for chemical analysis of most concerned Ah-agonists, i.e. polychlorinated dibenzo-p-dioxins (PCDDs), dibenzofurans (PCDFs), biphenyls (PCBs), and polycyclic aromatic hydrocarbons (PAHs). All raw sediment extracts induced significant Ah-agonistic effects, and the bioassay-derived 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) equivalents of raw extracts (TEQ (raw) s) ranged from 8.5 to 336.0 pg/g dry weight (dw). Chemical analysis-derived TEQs (TEQ (chem) s) ranged from 20.5 to 64.8 pg/g dw. When raw extracts were purified by sulphuric acid silica gel column to derive acid stable fraction, the TCDD equivalents in this fraction (TEQ (stable) s) ranged from 2.7 to 63.8 pg/g dw. PCBs, PCDDs and PCDFs contributed about 51.4-72.1%, 1.2-7.3%, and 16.4-34.8% of TEQ (stable) s, respectively, and the sum was 69.1-108.6%. Therefore, about 65.0% of TEQ (raw) s could be attributed to acid labile fraction and only 3.3-9.6% to PAHs. These observations suggested that acid stable fraction and labile fraction contributed together to total Ah-agonistic effects in the sediments, and PCBs and PCDFs might be the two main components in acid stable Ah-agonists. The proposed approach using both H4IIE cell bioassay and chemical analysis could be used for characterization and prioritization of Ah-agonists in river sediments and helpful to the following progression of ecological remediation. |
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