Protein Information

ID 2608
Name Cr 3
Synonyms CR 3; CRIPTO; TDGF 1; TDGF1; TDGF 2; TDGF2; CRIPTO 3; CRIPTO3…

Compound Information

ID 1409
Name silica gel
CAS silica gel

Reference

PubMed Abstract RScore(About this table)
18681457 Lafleur JP, Salin ED: Speciation of chromium by high-performance thin-layer chromatography with direct determination by laser ablation inductively coupled plasma mass spectrometry. Anal Chem. 2008 Sep 1;80(17):6821-3. Epub 2008 Aug 6.
It is of considerable importance to be able to distinguish metallic species because their toxicity depends on their chemical form. Therefore, the analysis of environmental samples can be enhanced by the combination of high-performance thin-layer chromatography (HPTLC) with laser ablation inductively coupled plasma mass spectrometry (LA-ICPMS). In this study, Cr (3+) and Cr (6+) were separated on silica gel HPTLC plates using aqueous mobile phases. Separation was achieved in seconds with retardation factors ( R f ) of 0 and 1 for Cr (3+) and Cr (6+), respectively. LA was used to volatilize the chromium species directly from the chromatographic material prior to ICPMS detection. A linear calibration was obtained, and detection limits (3sigma) of 6 ng for Cr (6+) and 0.4 ng for Cr (3+) were achieved with precision ranging from 3 to 40% at the 95% confidence level. The silicon present in the stationary phase was used as an internal standard. This procedure allows for a rapid separation and quantification, requires only 0.5 muL of sample, and lower detection limits can be achieved through preconcentration.
8(0,0,1,3)