Protein Information

ID 59
Name alkaline phosphatase
Synonyms ALP 1; Alkaline phosphatase; ALPG; ALPPL; ALPPL 2; ALPPL2; GCAP; Germ cell alkaline phosphatase…

Compound Information

ID 1328
Name nicotine
CAS

Reference

PubMed Abstract RScore(About this table)
19927714 Bai B, Zhong LF, Zhu JT: [Nicotine affects biological properties of the human osteosarcoma cell line MG63]. Hua Xi Kou Qiang Yi Xue Za Zhi. 2009 Oct;27(5):483-6.
OBJECTIVE: To evaluate the effect of nicotine on cell proliferation. METHODS: The cells were cultured with DMEM medium containing 10% fetal bovine serum with 0, 1 x 10 (-4) mol x L (-1), and 1 x 10 (-3) mol x L (-1) nicotine for up to 3, 5, 7, 10, 14 days. The cell proliferation was evaluated by MTT. The alkaline phosphatase (ALP) activity was estimated by PNPP. The expression of collagen type I (COL1) and osteocalcin (OCN) were estimated by reverse transcription-polymerase chain reaction (RT-PCR). RESULTS: Nicotine suppressed the cell proliferation. ALP activity increased to peak on 10 days in control and 1 x 10 (-4) mol x L (-1) nicotine. COL1 expression increased to peak on 10 days in control and 1 x 10 (-4) mol x L (-1) nicotine, but there was decreased to the minimum on 10 days and increased on 14 days in 1 x 10 (-3) mol x L (-1) nicotine. OCN expression increased to peak on 10 days in control, and increased in 1 x 10 (-4) mol x L (-1) nicontine from 3 days to 14 days, but there wasn't significant change in 1 x 10 (-3) mol x L (-1) nicontine. CONCLUSION: Nicotine suppresses osteogenesis through a decrease in ALP and COL1 production by osteoblasts.
1(0,0,0,1)