| 15702242 |
Mihaly A, Borbely S, Vilagi I, Detari L, Weiczner R, Zador Z, Krisztin-Peva B, Bagosi A, Kopniczky Z, Zador E: Neocortical c-fos mRNA transcription in repeated, brief, acute seizures: is c-fos a coincidence detector?. Int J Mol Med. 2005 Mar;15(3):481-6.
The effect of acute brief seizures on neocortical c-fos expression was investigated in rats injected with 5 mg/kg 4-aminopyridine. Electroencephalography in freely moving animals with implanted neocortical electrodes detected an average of 2.67 tonic-clonic convulsions within 1 h following the 4-AP treatment. Tissue samples of the somatosensory neocortex were collected at 30 min, 1 h, 3 h, 5 h and 8 h following the treatment for PCR and immunohistochemistry. The c-fos mRNA displayed the first significant rise at 1 h, and remained significantly higher through 3 h. The number of c-fos protein immunoreactive cells was significantly elevated already at 30 min, peaked at 1 h, and declined by 5 h. We conclude that in repetitive, brief seizures, the first convulsion does not increase c-fos RNA transcription, whilst the second causes a long-lasting gene expression and a large increase of c-fos protein synthesis. The phenomenon may have implications in the pathogenesis of human and animal epilepsies. |
9(0,0,1,4) |