| 10725560 |
Cheong YH, Kim1 CY, Chun HJ, Moon BC, Park HC, Kim JK, Lee S, Han C, Lee SY, Cho MJ: Molecular cloning of a soybean class III beta-1,3-glucanase gene that is regulated both developmentally and in response to pathogen infection. Plant Sci. 2000 May 15;154(1):71-81.
We isolated and characterized a soybean gene (SGN1) encoding a basic beta-1,3-glucanase that is a plant class III isoform of beta-1,3-glucanase. The deduced amino acid sequence of the SGN1 gene is similar to that of the PR-Q'b gene, the basic class III beta-1,3-glucanase of tomato. Based on RNA blot hybridization, SGN1 gene expression was detected in all tissues of 4-day old seedlings, but it was present only in root tissue of 30-day old plants. GUS expression analysis carried out in transgenic tobacco plants harboring a SGN1::GUS reporter gene revealed the same expression pattern. Furthermore, the expression of SGN1 was strongly induced by a variety of defense-related signals, such as treatment with H (2) O (2), wounding, or treatment with fungal elicitor prepared from Phytophthora spp as well as inoculation with Pseudomonas syringae. However, the expression level of SGN1 was hardly induced with jasmonate, ethephon and salicylate. Overall the results suggest that the SGN1 may play a role in both plant development and plant defense against pathogen attack. |
11(0,0,1,6) |