| 19548584 |
Qin H, Li Y, Shi L, Sun C: [Effect of t10, c12-conjugated linoleic acid on fatty acid metabolism in C2C12 myotubes]. Wei Sheng Yan Jiu. 2009 May;38(3):355-8.
OBJECTIVE: To study the effect of t10, c12-conjugated linoleic acid (t10, c12-CLA) on fatty acid metabolism in myotubes under the high free fatty acid condition. METHODS: We used palmitate to simulate a high free fatty acid condition for C2C12 myotubes in vitro and administered t10, c12-CLA across the groups. The expression and translocation of FAT/CD36 were determined by fluorimetric immunifaction using laser scanning confocal microscope. A series of enzymes related to fatty acid metabolism, together with AMP-activated protein kinase (AMPK), were determined by western blot. RESULTS: t10, c12-CLA dose-dependently increased the translocation of FAT/CD36 to cellular membranes, and increased the expression of p-ACC and CPT-1 in myotubes under the high free fatty acid condition. Moreover, t10, c12-CLA activated the AMPK in myotubes. CONCLUSION: CLA can increased the fatty acid oxidation in myotubes via activating AMPK. |
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