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Ding L, Zhu S, Xie S, Wu X: Effect of exogenous bFGF on the proliferation of human adenoid cystic carcinoma ACC-2 cells. J Huazhong Univ Sci Technolog Med Sci. 2008 Apr;28(2):227-9. Epub 2008 May 15.
To observe the effects of basic fibroblast growth factor (bFGF) on human adenoid cystic carcinoma ACC-2 cell line proliferation and ERK, cyclin D1/p21 (waf/cip1) signaling pathways, human adenoid cystic carcinoma cells (ACC-2) were cultured and the influence of bFGF of different concentrations on cell proliferation was determined by MTT. Protein was detected by immuno-precipitation and ERK activity by using ERK agent kit. p-ERK (1/2) and down-stream cyclin D1, p21 (waf/cip1) expression were detected by Western blotting and the interfering role of mitogen protein-activated kinase (MEK) suppressor U0126 in the afore-mentioned indicators was examined. MTT demonstrated ACC-2 cell proliferation was substantially enhanced by bFGF, immuno-precipitation displayed ERK activity was up-regulated by bFGF, and immuno-imprinting also showed p-ERK (1/2), cyclin D1 expression was greatly enhanced and p21 (waf/cip1) expression was inhibited by bFGF. U0126 suppressed the effect of bFGF. It is concluded that bFGF can promote the proliferation of human adenoid cystic carcinoma ACC-2 cells, and its pathways are associated with the up-regulated activity and expression of p-ERK (1/2), inhibited p21waf/cip1 expression and enhanced cyclin D1 expression. |
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