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Nataraja K, Neelambika GK: Somatic embryogenesis and plantlet from petal cultures of pomegranate, Punica granatum L. Indian J Exp Biol. 1996 Jul;34(7):719-21.
Somatic embryos were induced through petal cultures on MS supplemented with various growth adjuvants individually or in different combinations. Numerous embryoids formed from friable callus on MS with 5 mg/L of IAA, IBA or NAA developed only roots, but those formed on MS with 5 mg/L of BAP or KN developed shoots. Explants reared on MS supplemented with IAA (5 mg/L) and BAP (5 mg/L) though differentiated numerous embryoids but developed multilobed or fused cotyledons. However, callus induced on MS with IAA (1 mg/L) or IBA (1 mg/L) on subculturing in the same medium with half-strength salts and sucrose (4%) produced both roots and shoots resulting in plantlets. |
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