| 14730895 |
Zhang JJ, Shi RL: [Effects of caspases on cerebromicrovascular endothelial cell apoptosis induced by hypoxia]. Yao Xue Xue Bao. 2003 Oct;38(10):739-42.
AIM: To study the effects of caspases on cerebromicrovascular endothelial cell apoptosis induced by hypoxia in vitro. METHODS: The cultured bovine cerebromicrovascular endothelial cells were exposed to NaCN in glucose-free medium. Cell viability was determined by trypan blue staining. Cell apoptosis was defined by terminal deoxynucleotidyl transferase-mediated dUTP nick end-labeling (TUNEL) and flow cytometry. The expression of caspase-3 was detected by immunocytochemical method. Four caspase inhibitors were used to validate the effect of caspases on cell apoptosis. RESULTS: NaCN in glucose-free medium initiated cerebromicrovascular endothelial cell injury markedly and typical apoptotic cells were found in this model. The expression of caspase-3 increased significantly. Four caspase inhibitors decreased the number of injured cells. Selective inhibitor of caspase-1 and -6 reduced expression of caspase-3 significantly. CONCLUSION: The results suggest that caspases family plays an important role in cerebromicrovascular endothelial cell apoptosis induced by NaCN and caspase-3 acts on the downstream of caspase-1 and -6 in protease cascade action to induce apoptosis. |
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