| 8100588 |
Lonart G, Cassels KL, Johnson KM: Nitric oxide induces calcium-dependent [3H] dopamine release from striatal slices. J Neurosci Res. 1993 Jun 1;35(2):192-8.
Hydroxylamine (0.01-30 mM), a nitric oxide (NO) generator, produced a concentration-dependent release of [3H] dopamine ([3H] DA) from rat striatal slices. Hemoglobin (10 microM), a NO scavenger, reduced basal [3H] DA release and blocked hydroxylamine (100 microM)-stimulated [3H] DA efflux. Tetrodotoxin (0.5 microM) had no significant effect. Sodium cyanide was used as a model compound to test the possibility that NO acted through blockade of mitochondrial electron transport. Calcium-free experimental buffer (1 mM EGTA) reduced basal release and the hydroxylamine response, while sodium cyanide-induced release did not change under these experimental conditions. Cadmium (200 microM), a non-selective inhibitor of voltage-dependent calcium channels, reduced the hydroxylamine response by 69%. Methylene blue (10 microM), an inhibitor of guanylate cyclase, produced a 3-fold increase in the basal release but had no significant effect on the hydroxylamine response. These data suggest that NO induces calcium-dependent [3H] DA release from the striatum via a mechanism which is independent of blockade of electron transport or activation of guanylate cyclase. |
0(0,0,0,0) |