| 20346998 |
Maran E, Fernandez-Franzon M, Font G, Ruiz MJ: Effects of aldicarb and propoxur on cytotoxicity and lipid peroxidation in CHO-K1 cells. Food Chem Toxicol. 2010 Mar 24.
Cytotoxic effects of aldicarb, its sulfone and sulfoxide, and propoxur, lipid peroxidation and antioxidant parameters in Chinese Hamster Ovary (CHO-K1) cells were determined. d,l-buthionine-(S,R)-sulfoximine (BSO) was assayed to determine the role of GSH in the protection against carbamate cytotoxicity. Pretreatment with 60 muM BSO, induced a significant decrease in the glutathione reductase (GR; 64 to 141%), the glutathione peroxidase (GPx; 10 to 30%) and the glutathione S-transferase (GST; 59 to 93%) activities, and it GSH levels (79-85%), while the oxidized glutathione (GSSG) levels significantly increased (64-78%) respect to experiment non BSO-pretreated. Carbamates BSO-pretreated vs. non BSO-pretreated showed a significant increase in malondialdehyde (MDA) production (from 13 to 52% vs. 25 to 93%). These data suggest that carbamates could injure CHO-K1 cells via oxidative stress by the increase of MDA production; moreover, BSO enhance the oxidative damage caused by carbamates. However, the glutathione system protects cells from carbamates damage. |
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