| 18051317 |
Park J, Kim D, Kim S, Kim J, Bae K, Lee C: The analysis and application of a recombinant monooxygenase library as a biocatalyst for the Baeyer-Villiger reaction. J Microbiol Biotechnol. 2007 Jul;17(7):1083-9.
Because of their selectivity and catalytic efficiency, BVMOs are highly valuable biocatalysts for the chemoenzymatic synthesis of a broad range of useful compounds. In this study, we investigated the microbial Baeyer-Villiger oxidation and sulfoxidation of thioanisole and bicyclo [3.2.0] hept-2-en-6-one using whole Escherichia coli cells that recombined with each of the Baeyer-Villiger monooxygenases originated from Pseudomonas aeruginosa PAO1 and two from Streptomyces coelicolor A3 (2). The three BVMOs were identified in the microbial genome database by a recently described protein sequence motif; e.g., BVMO motif (FXGXXXHXXXW). The reaction products were identified as (R)-/(S) sulfoxide and 2-oxabicyclo/3-oxabicyclo [3.3.0] oct-6-en-2-one by GC-MS analysis. Consequently, this study demonstrated that the three enzymes can indeed catalyze the Baeyer-Villiger reaction as a biocatalyst, and effective annotation tools can be efficiently exploited as a source of novel BVMOs. |
31(0,1,1,1) |