| 11479384 |
Cassan FD, Lucangeli CD, Bottini R, Piccoli PN: Azospirillum spp. metabolize [17,17-2H2] gibberellin A20 to [17,17-2H2] gibberellin A1 in vivo in dy rice mutant seedlings. Plant Cell Physiol. 2001 Jul;42(7):763-7.
Azospirillum spp. are endophytic bacteria with beneficial effects on cereals--effects partially attributed to gibberellin production by the microorganisms. Azospirillum lipoferum and Azospirillum brasilense inoculated to rice dy mutant reversed dwarfism in seedlings incubated with [17,17-2H2] GA20 with formation of [17,17-2H2] GA1, showing the in vivo capacity to perform the 3beta-hydroxylation. When prohexadione-Ca, an inhibitor of late steps in gibberellin biosynthesis, was added to the culture medium, no complementation was observed and no [17,17-2H2] GA1 was produced. The latter suggests that the bacterial operating enzyme may be a 2-oxoglutarate-dependent dioxygenase, similar to those of plants. |
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