| 19185358 |
Na N, Guo H, Zhang S, Li Z, Yin L: In vitro and in vivo acute toxicity of fenpyroximate to flounder Paralichthys olivaceus and its gill cell line FG. Aquat Toxicol. 2009 Apr 9;92(2):76-85. Epub 2008 Dec 27.
Fenpyroximate, an acaricide, is widely used in the prevention of acarids (mites) in plants. In this study, the in vitro and in vivo acute toxicity of fenpyroximate was examined using the marine flounder Paralichthys olivaceus and its gill cell line (FG). The 48h-IC (50) (95% confidence limits) values of fenpyroximate in the FG cells were 890 (790-990) nM, 950 (881-1019) nM and 1250 (1159-1341) nM, and 96h-IC (50) (95% confidence limits) were 480 (388-572) nM, 490 (454-526) nM and 510 (469-551) nM, for methyl thiazolyl tetrazolium (MTT) assay, neutral red (NR) uptake and cell protein assay, respectively. The 48h- and 96h-LC (50) (95% confidence limits) values of fenpyroximate in living flounders were 28.84 (14.28-58.26) nM and 11.74 (6.06-22.8) nM, respectively. This indicated that fenpyroximate was highly toxic to both flounders and FG cells. Moreover, comparisons of the ratios of average 48h-IC (50) to 48h-LC (50) and average 96h-IC (50) to 96h-LC (50) showed that the length of exposure time did not significantly affect the correlation between the FG cells and living flounders in the acute toxicity estimation of fenpyroximate provided the selected exposure time is the same. Thus, we suggest that FG cells could be a good bioassay system in rapid estimation of the corresponding LC (50) values of pollutants to living fish, instead of whole living fish. Histopathological examinations showed that liver and gill were the major target organs of fenpyroximate, especially the damage of gill tissues may account much for the high lethality of exposed flounders. Consistent with the histopathological observations, analysis of the activities of two key detoxification metabolism-related enzymes, glutathione S-transferase (GST) and cytochrome P4501A1 (CYP1A1)-dependent ethoxyresorufin-O-deethylase (EROD), in the liver and gill tissues of exposed flounders indicated that liver has much higher detoxification capacity than gills, and this contributes to the higher tolerance of liver to the toxicity of fenpyroximate in the exposed flounders. Fenpyroximate can initially induce a quick and significant increase of the activities of the antioxidant enzymes superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPX) in all the exposed FG cells, and liver and gill tissues of exposed flounders. Upon continuation of the exposure the enzyme activities were inhibited, implying the occurrence of oxidative stress in the exposed fish cells and the possible interruption of the mitochondrial respiratory chain which involves redox reactions by fenpyroximate. |
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