| 20200125 |
Janssen D, Derst C, Rigo JM, Van Kerkhove E: Cys-loop ligand-gated chloride channels in Dorsal Unpaired Median neurons of Locusta migratoria. J Neurophysiol. 2010 Mar 3.
In insects, inhibitory neurotransmission is generally associated with members of the cys-loop ligand-gated anion channels, such as the glutamate-gated chloride channel (GluCl), the gamma-amino butyric acid-gated chloride channels (GABACl) and the histamine-gated chloride channels (HisCl). These ionotropic receptors are considered being established target sites for the development of insecticides and therefore, it is necessary to obtain a better insight in their distribution, structure and functional properties. Here, by combining electrophysiology and molecular biology techniques, we have identified and characterized GluCl, GABACl and HisCl in DUM neurons of Locust migratoria. In whole-cell patch-clamp recordings, application of glutamate, GABA or histamine induced rapidly activating ionic currents. GluCls were sensitive to ibotenate and blocked by picrotoxin and fipronil. The pharmacological profile of the L. migratoria GABACl fitted neither the vertebrate GABAA nor GABAC receptor and was similar to the properties of the cloned Drosophila melanogaster GABA receptor subunit (Rdl). The expression of Rdl-like subunit-containing GABA receptors was demonstrated at the molecular level using RT-PCR. Sequencing analysis indicated that the orthologous GABACl of D. melanogaster CG10357-A is expressed in DUM neurons of L. migratoria. Histamine-induced currents exhibited a fast onset and desensitized completely on continuous application of histamine. In conclusion, within the DUM neurons of L. migratoria, we identified three different cys-loop ligand-gated anion channels that use GABA, glutamate or histamine as their neurotransmitter. |
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